Introduction Painting pigments and mediums have been around for centuries. This experiment was used to create pigments and a medium used by the ancients. Various pigments were made through reactions in the laboratory and, once dry, the means to create individual paintings were created. Some produced acrylic or watercolor and others produced egg tempera or casein. Acrylic paint was developed in the 1940s and is a relatively new medium. As an alternative to the long drying time of oil paint, quick-drying acrylic paint became a pioneer in the 1950s. (arthistory.net, 2013) Acrylic is a common medium today, and due to its quick-drying capabilities, it is not easily blended. However, it is a very popular medium (Figure 1). Gum arabic or watercolor is one of the oldest mediums, dating back approximately 5000 years to the ancient Egyptians. Gum arabic, also called gum acacia, comes from unhealthy acacia trees. The more damaged the tree, the more gum can be harvested. (iscgums.com) Gum arabic, like acrylic, is a very popular form to paint with and although it is more difficult due to longer drying times, it produces beautiful paintings (Figure 2). Egg tempera dates back to the 12th century and is now a widely available commercial paint. Today it is often found in elementary schools and was used for medieval panel paintings when egg yolks were added to the tempera formula. With the consistency of egg tempera and the invention of synthetic materials, it became an unfavorable medium. However, it still produces beautiful colors (figure 3) and is used by new artists or those looking to try different mediums. (arthistory.net, 2013) Egg tempera is more resistant as a paint than oil. It dries hard and supple, making it more durable than other media. (Jusko, ?) It turned out that casein paint, used in this experiment, was used in ancient times