GOOD LABORATORY PRACTICES While carrying out practices in any laboratory we must strictly follow the rules and adopt good laboratory practices. Some of the instructions that everyone should follow are discussed below. SAFETY IN THE MICROBIOLOGY LABORATORY The techniques and microorganisms involved in the practice must present a minimum risk for workers. It is therefore necessary to always adopt good practices when working with microorganisms because they can be potentially pathogenic. All equipment and culture media must be properly sterilized before performing the experiment. Preparation of the mother culture for the preparation of the inoculum. Prepared culture media inoculated with prepared inoculum. The inoculated media are incubated and sampled. Safe disposal of cultures after sterilization. PROTECTION No food should be brought into or consumed within a microbiology laboratory. Avoid contact with objects such as pencils, labels, pens etc. with the mouth in the laboratory . Hands must be sterilized immediately after carrying out the practice with a disinfectant/disinfectant. Cuts and wounds must be protected adequately with bandages. All experiments must be carried out after adequate guidance and under the supervision of the person concerned. ASEPTIC TECHNIQUES Equipment must be sterile during culture and transfer of microbes to avoid any contamination. Used or contaminated equipment must be autoclaved. ELECTRICAL SAFETY Many equipment such as bioreactor, scale, stirrer etc. they run on electricity nowadays. No contact of such equipment with formic-type liquids must be guaranteed. GLASS Glassware must be handled and sterilized properly. In case of accidents... half the paper... not drinkable. REFERENCES Balasubramanyam MK, BiE, Glotzer. M (2004).“ Comparative analysis of cytokinesis in budding yeast”“Brewer's yeast”, University of Maryland, medical center.Erhard Friedrich Leuchs (1831) “Wirkung des speichels auf starke.Poggendorff'sahnalen der physik und chemie, volume 22, page 623.Flickers P, Benelti PH, Wache Y, Marty A, Mauersberger S Smith MS, Nicaud, JM (2005) “Hydrophobic substrate utilization by the yeast Yarrowialipolytica and its potential applications.” FEMS research on yeastHistory of biology 1800-1849.Huxley A (1871). “Discourses: biological and geological (VolumeIII): Yeast”. Collected essays.Kurtzman, C P. 2006Legras JL, Merdinogln D, Cornnet JM, Karst F. (2007). “Bread, beer and wine: the diversity of Saccharomyces cerevisiae reflects human history. Robert Hill and Joseph Needham. The chemistry of life.