Chitosan nanoparticles have been widely used as drug delivery systems in recent years. In the present study, chitosan nanoparticles loaded with Echis carenataus snake venom were prepared based on the ionic gelation of tripolyphosphate and chitosan. The physicochemical characteristics of the nanoparticles, the stability and biological activity of the encapsulated venom were studied. The particles were spherical in shape and the tripolyphosphorus groups of TPP were bonded to the ammonium groups of chitosan. The optimal particle size of chitosan and venom-loaded nanoparticles was 89 and 116 nm, respectively. Optimal loading capacity and loading efficiency achieved with a poison concentration of 500 µg/ml. The biological activity of the venom remained intact during nanparticulation and did not show any considerable reduction in the stability analysis. Our results suggest that chitosan nanoparticles, prepared in this work, could be used as an alternative to traditional adjuvants. Keywords: chitosan nanoparticles, tripolyphosphate, poison, ionic gelation, stability. Chitosan nanoparticles due to biocompatibility...