The aim of the present study was to examine the effects of long-term/exhaustive physical exercise and spirulina intake on the oxidative status of plasma, liver and muscle tissues in adult male rats. We found that exhaustive exercise led to an increase in plasma CK, CK-MB, and LDH levels in group E. An important finding of our study is that spirulina attenuated the exercise-induced exhaustive increase in CK in the plasma. Furthermore, spirulina supplementation and acute or chronic exercise modified antioxidant status and oxidative stress parameters. MDA, which is an end product of lipid peroxidation, was decreased in both spirulina and chronically exercised rats in the liver and skeletal muscle. We observed that the most important effects related to oxidative stress are associated with adaptive responses related to regular physical exercise. Although the influence of exercise on metabolism is well established, the rats' weight gain was no different compared to control rats. This result is in agreement with previous studies (barcelos24334002, lima23405192). However, it can be suggested that swimming exercise does not have a major impact on the weight of rats, probably due to the intensity and duration of the exercise. Furthermore, supplementation of 750 mg/kg/day pospirulin for 6 weeks did not alter the weight of rats compared to control animals. We have not found any studies in the medical literature reporting the direct effects of spirulina on body weight. However, there are several studies highlighting how spirulina influences weight (ismail19521547, khan16858688, moura21569626) which are in line with our findings. So, in summary, it can be deduced that spirulina supplementation has no effect on the weight of rats. Moderate exercise ...... middle of paper ...... 688) and gentamisin-induced (Kuhadve ark 2006- 16573712) oxidative stress in kidney tissue. In conclusion, we suggest that spirulina platensis, a blue-green algae and dietary supplement, enhances plasma CK activity which is used as a comprehensive indicator of exercise-related muscle breakdown, probably by decreasing preoxidative MDA levels in the renal tissue. skeletal muscle. However, it is evident that spirulina supplementation in adult male rats has no positive effect on antioxidant activity and ergogenic state in our exercise protocol. There are few studies investigating the effects of spirulina supplementation on exercise-induced oxidative stress and exercise endurance capacity, and the mechanism of action of spirulina on oxidative stress during exercise processes is still not well understood. including, while future studies in this area are needed to have a satisfactory result. comprehension.