In orthopedic surgery, the iliac bone graft, commonly used to treat nonunion or spinal fusion, contains multipotential stem cells or mesenchymal progenitor cells. These cells are found approximately 1 in 10,000 nucleated cells in the bone marrow. Friedenstein et al. (1970) reported that a rare population of cells with the ability to adhere to plastic was found (Friedenstein et al., 1970). These cells are capable of proliferation and differentiation of mesenchymal lineages (Pittenger et al., 1999, Caplan, 1991). The unique antigens of MSCs (hMSCs) are controversial, and the limited popularity of cell sorting techniques are not as widely used as gradient media in MSC isolation. Most MSC populations were isolated due to their ability to adhere to plastic, similar to that originally used by Friedenstein. The direct BM plating method is commonly used for small animal cells (Lennon and Caplan, 2006, Nadri et al., 2007), but in human bone marrow, density gradient centrifugation is the most commonly used method to isolate MSC. So far, it is still controversy to exactly identify MSCs. Based on their capacity for multilineage proliferation and differentiation, the colony-forming fibroblast-unit (CFU-F) is defined as highly adherent in a colony of fibroblast-like cells that arise from a parent cell. Therefore, the CFU-F assay was used to evaluate bone marrow progenitors and the number of colonies formed per total number of seeded marrow cells or colony formation efficiency (CFE), thus the CFU-F content among marrow cells, representing in vitro enumeration of a clonogenic subset of marrow stromal cell populations in vivo. However, it has been shown that CFU-F populations are not homogeneous but rather contain a hierarchy of progenitors including multipotential mesechymal stem cells and committed progenitors ( Friedenstein et al., 1992 , Latsinik et al., 1986 ). Several surface producers expressing from mesenchymal stem cells. Basically, these producers are cell receptors, adhesion molecules, extracellular matrix proteins, cytokines and other molecules that have the function of communicating with other cells and carrying out their physiological functions. Therefore, these manufacturers are adopted to characterize homogeneous mesenchymal stem cells. However, bone marrow-derived stem cell expression of several surface markers is still controversial. These cells have a negative expression of CD45 which is expressed in hematopoietic stem cells (HSCs) (McKinney-Freeman et al., 2009), CD14 which is expressed in innate immune cells (Cros et al...